Identification of Trichophyton spp. Fungi on Farmers' Toenails in Jagalempeni Village, Brebes Regency
DOI:
https://doi.org/10.54783/influencejournal.v8i2.364Keywords:
Trichophyton Rubrum; Trichophyton Mentagrophytes; Farmers; Dermatophytes; Sabouraud Dextrose Agar.Abstract
Tinea unguium is a fungal infection caused by dermatophytes and frequently affects individuals in high-risk occupations, such as farmers. Humid working environments, direct contact with soil, and working barefoot increase the risk of fungal nail infections. This study aimed to identify the fungal species causing tinea unguium in the toenails of farmers in Jagalempeni Village, Wanasari District, Brebes Regency, Indonesia. A descriptive cross-sectional study was conducted using 15 toenail scraping specimens cultured on Sabouraud Dextrose Agar (SDA) and identified microscopically with Lactophenol Cotton Blue (LPCB) staining. The results showed the presence of both dermatophyte and non-dermatophyte fungi. Dermatophytes included Trichophyton rubrum (40%) and Trichophyton mentagrophytes (20%), while non-dermatophytes comprised Aspergillus spp. (20%), Rhizopus spp. (13%), and Candida spp. (7%). Trichophyton rubrum was the predominant causative agent. These findings indicate that humid working conditions, prolonged farming activities, and poor foot hygiene may increase the risk of fungal nail infections. Improving personal hygiene and using appropriate protective footwear are recommended to reduce the incidence of tinea unguium among farmers.
References
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Page, J. H., Sebayang, R., & Kurniawan, I. (2021). Perbedaan jumlah koloni jamur Trichophyton rubrum pada media Sabouraud Dextrose Agar dan modifikasi glukosa 3 g. Jurnal Penelitian Sains, 23(3), 134–139.
Prabandari, A. S., Pramonodjati, F., & Sari, A. N. (2024). Isolasi dan identifikasi jamur penyebab onikomikosis pada petani di Kabupaten Sukoharjo, Jawa Tengah. Indonesian Journal on Medical Science, 11(1).
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Salsabilla, D. P., Bedah, S., & P. E. (2025). Epidemiologi infeksi jamur: Kasus tinea pedis di kalangan pencuci jasa kendaraan bermotor. Jurnal Fatmawati Laboratory & Medical Science, 5(1), 25–37.
Alyatul Himma, Mufida, D. C., Sofiana, K. D., Armiyanti, Y., & A. D. (2024). The relationship between personal hygiene and the prevalence of tinea unguium infection among farmers in Jember District. Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan, 18(1), 44–50.
Amaliyah, S., & Rahmawati, A. (2023). Karakteristik jamur penyebab tinea unguium pada pedagang ikan. Jurnal Poltekkesmu, 8, 20–25.
Annisa, I., Zahra, M. A., & Prihati, M. (2025). Fungi dermatofita pada kuku kaki petani di Desa Sumurbandung Kabupaten Lebak. Journal of Medical Laboratory, 3(1), 33–37.
Apridamayanti, A. G. A. R. S. P. (2021). Uji karakteristik makroskopik jamur dermatofita yang terdapat pada ulkus diabetikum derajat III dan IV Wagner. Jurnal Farmasi Kalbar, 4(1), 11.
Iftinan, K., Raharjo, A. M., Agustina, D., & Mufida, D. C. (2025). Personal protective footwear and the risk of tinea unguium among Lojejer villager farmers. Indonesian Journal of Occupational Safety and Health, 14(2), 222–229. https://doi.org/10.20473/ijosh.v14i2.2025.222-229
Khasanah, M., Marsudi, L. O., & Penelitian, M. (2021). Studi literature agen dan faktor risiko penyebab tinea unguium pada infeksi kuku kaki petani. Jurnal Teknologi Laboratorium Medik Borneo, 1(1), 34–41.
Khodadadi, H., Zomorodian, K., Nouraei, H., Zareshahrabadi, Z., Barzegar, S., Reza, M., & Keyvan, Z. (2021). Prevalence of superficial cutaneous fungal infections in Shiraz, Iran: A five-year retrospective study (2015–2019). Journal of Clinical Laboratory Analysis. https://doi.org/10.1002/jcla.23850
Lubis, S. S., & Nacia, A. A. (2025). Description of tinea unguium infection levels in farmers' nails in Meunasah Pupu Village, Ulim District, Pidie Jaya Regency. International Journal of Science, Technology and Applications, 3(1), 12–24.
Mushlih, M. (2025). Dermatophyte fungi causing tinea unguium in toenails of builders in Bangkalan. Indonesian Journal on Health Science and Medicine, 1–11.
Nadifah, F., Muhajir, N. F., Sanusi, K. E., Nuryati, A., & Murtiningrum, S. (2025). Identifikasi jamur penyebab tinea unguium pada kuku kaki petani di Dusun Baros, Tirtohargo, Kretek, Bantul. Jurnal Biosense, 8(3).
Nurdin, E., Zuchrullah, M., & M. A. N. I. (2023). Deteksi jamur dermatofit dan non-dermatofit pada tinea unguium menggunakan media alternatif sukun dextrose agar. Jurnal Inovasi Pendidikan dan Sains, 4(1), 16–20.
Page, J. H., Sebayang, R., & Kurniawan, I. (2021). Perbedaan jumlah koloni jamur Trichophyton rubrum pada media Sabouraud Dextrose Agar dan modifikasi glukosa 3 g. Jurnal Penelitian Sains, 23(3), 134–139.
Prabandari, A. S., Pramonodjati, F., & Sari, A. N. (2024). Isolasi dan identifikasi jamur penyebab onikomikosis pada petani di Kabupaten Sukoharjo, Jawa Tengah. Indonesian Journal on Medical Science, 11(1).
Rachmawati, F., Nursidika, P., & Fitrianingsih, P. (n.d.). Identifikasi jamur Trichophyton sp. penyebab tinea unguium pada petani di Desa Mekarluyu, Kabupaten Garut. Jurnal Penelitian Saintek, 27(2), 112–118.
Salsabilla, D. P., Bedah, S., & P. E. (2025). Epidemiologi infeksi jamur: Kasus tinea pedis di kalangan pencuci jasa kendaraan bermotor. Jurnal Fatmawati Laboratory & Medical Science, 5(1), 25–37.
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